Assay for optical determination of biogenic amines using microtiterplates

Polona Nedeljko; Matejka Turel; Aleksandra Lobnik

doi:10.1117/12.2019486

3 May 2013 Assay for optical determination of biogenic amines using microtiterplates

Polona Nedeljko, Matejka Turel, Aleksandra Lobnik

Author Affiliations +

Proceedings Volume 8774, Optical Sensors 2013; 87741A (2013) https://doi.org/10.1117/12.2019486
Event: SPIE Optics + Optoelectronics, 2013, Prague, Czech Republic

Abstract

Direct determination of catecholamine noradreanaline (NOR) is presented using o-phthaldialdehyde (OPA) as an indicator reagent. The fluorescent assay in which OPA forms with NOR a fluorescent complex (OPA-NOR) can be monitored at neutral, physiological conditions (pH 7) and performed in microtiterplates. The determination of NOR is optimal in the concentration range from 4.0×10^-7 to 1.0×10^-5 M and limit of detection is 4.0×10^-7 M. The OPA-NOR complex maximum intensity is reached within 5 minutes. Dopamine and adrenaline could not be determined using the same approach.

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Polona Nedeljko, Matejka Turel, and Aleksandra Lobnik "Assay for optical determination of biogenic amines using microtiterplates ", Proc. SPIE 8774, Optical Sensors 2013, 87741A (3 May 2013); https://doi.org/10.1117/12.2019486

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