18 November 2013 Measuring the area of tear film break-up by image analysis software
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Proceedings Volume 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications; 8785AC (2013) https://doi.org/10.1117/12.2030431
Event: 8th Ibero American Optics Meeting/11th Latin American Meeting on Optics, Lasers, and Applications, 2013, Porto, Portugal
Abstract
Tear film breakup time (BUT) test only examines the first break in the tear film, but subsequent tear film events are not monitored. We present a method of measuring the area of breakup after the appearance of the first breakup by using open source software. Furthermore, the speed of the rupture was determined. 84 subjects participated in the study. 2 μl volume of 2% sodium fluorescein was instilled using a micropipette. The subject was seated behind a slit-lamp using a cobalt blue filter together with a Wratten 12 yellow filter. Then, the tear film was recorded by a camera attached to the slit lamp. 4 frames of each video was extracted, the first rupture (BUT_0), breakup after 1 second (BUT_1), rupture after 2 seconds (BUT_2) and breakup before the last blink (BUT_F). Open source software of measurement based on Java (NIH ImageJ) was used to measure the number of pixels in areas of breakup. These areas were divided by the area of exposed cornea to obtain the percentage of ruptures. Instantaneous breakup speed was calculated for second 1 as the difference between BUT_1 - BUT_0, whereas instant speed for second 2 was BUT_2 - BUT_1. Mean area of breakup obtained was: BUT_0 = 0.26%, BUT_1 = 0.48%, BUT_2 = 0.79% and BUT_F = 1.61%. Break speed was 0.22 area/sec for second 1 and 0.31 area/sec for second 2, showing a statistical difference between them (p = 0.007). Post BUT analysis may be easily monitoring with the aid of this software.
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Hugo Pena-Verdeal, Hugo Pena-Verdeal, Carlos García-Resúa, Carlos García-Resúa, Lucía Ramos, Lucía Ramos, Antonio Mosquera, Antonio Mosquera, Eva Yebra-Pimentel, Eva Yebra-Pimentel, María Jesús Giráldez, María Jesús Giráldez, } "Measuring the area of tear film break-up by image analysis software", Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 8785AC (18 November 2013); doi: 10.1117/12.2030431; https://doi.org/10.1117/12.2030431
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