Resolution enhancement and autofocusing in digital holographic microscopy by using structured illumination

Peng Gao; Giancarlo Pedrini; Wolfgang Osten

doi:10.1117/12.2020298

13 May 2013 Resolution enhancement and autofocusing in digital holographic microscopy by using structured illumination

Peng Gao; Giancarlo Pedrini; Wolfgang Osten

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Proceedings Volume 8788, Optical Measurement Systems for Industrial Inspection VIII; 878809 (2013) https://doi.org/10.1117/12.2020298
Event: SPIE Optical Metrology 2013, 2013, Munich, Germany

Abstract

In this paper we show how resolution enhancement and autofocusing in digital holographic microscopy (DHM) is obtained by using structured illumination. The structured illumination is generated by a spatial light modulator, and enables to project fringes of different orientations and phase shift without mechanical movement. The combination of the structured illumination and DHM improves the resolution of phase and amplitude imaging. Furthermore, the image plane is numerically determined by searching for the minimal deviation between the reconstructed images carried by different diffraction orders of the structured illuminations. The method can be applied to both amplitude and phase object.

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Peng Gao, Giancarlo Pedrini, Wolfgang Osten, "Resolution enhancement and autofocusing in digital holographic microscopy by using structured illumination", Proc. SPIE 8788, Optical Measurement Systems for Industrial Inspection VIII, 878809 (13 May 2013); doi: 10.1117/12.2020298; https://doi.org/10.1117/12.2020298

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