13 May 2013 Deconvolution microscopy of living cells for phase-contrast imaging
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Abstract
The goal of deconvolution microscopy for phase-contrast imaging is to reassign the optical blur to its original position and to reduce statistical noise, thus visualizing the cellular structures of living cells in three dimensions and at subresolution scale. The major features of this technology for a phase-contrast microscopy are discussed through a series of theoretical analyses. A few of possible sources of aberrations and image degradation processes are presented. The theoretical and experimental results have shown that deconvolution microscopy can enhance resolution and contrast by either subtracting or reassigning out-of-focus blur.
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Guanxiao Cheng, Guanxiao Cheng, Ping Xu, Ping Xu, Zhilong Sun, Zhilong Sun, Chunquan Hong, Chunquan Hong, Zelin Li, Zelin Li, } "Deconvolution microscopy of living cells for phase-contrast imaging", Proc. SPIE 8789, Modeling Aspects in Optical Metrology IV, 87890F (13 May 2013); doi: 10.1117/12.2020372; https://doi.org/10.1117/12.2020372
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