23 May 2013 Investigation on 3D morphological changes of in vitro cells through digital holographic microscopy
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Abstract
We report the investigation of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps (QPMs) of biological cells by digital holographic microscopy (DHM), with the aim to analyze the 3D positions and 3D morphology together. We consider as test case for our tool the in vitro bull sperm head morphometry analysis. Extraction and measurement of various morphological parameters are performed by using two methods: the anisotropic diffusion filter, that is based on the Gaussian diffusivity function which allows more accuracy of the edge position, and the simple thresholding filter. In particular we consider the calculation of area, ellipticity, perimeter, major axis, minor axis and shape factor as a morphological parameter, instead, for the estimation of 3D position, we compute the centroid, the weighted centroid and the maximum phase values. A statistical analysis on a data set composed by N = 14 holograms relative to bovine spermatozoa and its reference holograms is reported.
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Pasquale Memmolo, Pasquale Memmolo, Lisa Miccio, Lisa Miccio, Francesco Merola, Francesco Merola, Paolo A. Netti, Paolo A. Netti, Giuseppe Coppola, Giuseppe Coppola, Pietro Ferraro, Pietro Ferraro, } "Investigation on 3D morphological changes of in vitro cells through digital holographic microscopy ", Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 87920R (23 May 2013); doi: 10.1117/12.2020570; https://doi.org/10.1117/12.2020570
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