In situ imaging of the mouse cochlea using two-photon microscopy

Xin Yang; Ye Pu; Demetri Psaltis; Konstantina M. Stankovic

doi:10.1117/12.2022523

23 May 2013 In situ imaging of the mouse cochlea using two-photon microscopy

Xin Yang, Ye Pu, Demetri Psaltis, Konstantina M. Stankovic

Proceedings Volume 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials; 87920T (2013) https://doi.org/10.1117/12.2022523
Event: SPIE Optical Metrology 2013, 2013, Munich, Germany

Abstract

Intracochlear imaging is of great interest clinically because cochlea is the central organ of hearing. However, intracochlear imaging is technologically challenging due to the cochlea’s small size and encasement in bone. The state-of- the-art imaging techniques are not adequate for high resolution cellular imaging to establish diagnosis without destroying the cochlea. We report in situ imaging of intact mouse cochlea using endogenous two-photon excitation fluorescence (TPEF) as the contrast mechanism. TPEF eliminates the need for exogenous labeling and eradicating the staining-induced artifacts. We used a natural, membranous opening into the cochlea, the round window, as the optical access to reach the organ of Corti, requiring no additional slicing or opening. Our approach provides the maximum non-invasiveness in the imaging process. TPEF exhibits strong contrast allowing deep imaging of mouse cochlea with cellular and even subcellular resolution. Inner hair cell, outer hair cell and supporting cell are clearly identifiable in TPEF images. Distinct morphological differences are observed between healthy and noise-exposed cochleae, allowing detection of specific, noise-induced pathologic changes. The TPEF images taken through the round window are correlated with the whole mount sections, verifying their reliability. Compared with one-photon excitation fluorescence (OPEF) confocal microscope and wide-field transmission microscope images taken under the same magnification and resolution, TPEF images demonstrate clear advantages in terms of sharpness, signal to noise ratio and contrast. These capabilities provide a working foundation for microendoscopy-based clinical diagnostics of sensorineural hearing loss.

Citation Download Citation

Xin Yang, Ye Pu, Demetri Psaltis, and Konstantina M. Stankovic "In situ imaging of the mouse cochlea using two-photon microscopy", Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 87920T (23 May 2013); https://doi.org/10.1117/12.2022523

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available