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17 June 2013 Changes induced by peripheral nerve injury in the morphology and nanomechanics of sensory neurons
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Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to this regenerative growth. In the present study, we use differential interference contrast microscopy, fluorescence microscopy and atomic force microscopy (AFM) to show that conditioned axotomy, induced by sciatic nerve injury, does not increase somatic size of sensory neurons from adult mice lumbar dorsal root ganglia but promotes the appearance of longer and larger neurites and growth cones. AFM on live neurons is also employed to investigate changes in morphology and membrane mechanical properties of somas of conditioned neurons following sciatic nerve injury. Mechanical analysis of the soma allows distinguishing neurons having a regenerative growth from control ones, although they show similar shapes and sizes.
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Ouafa Benzina, Vivien Szabo, Olivier Lucas, Marie-belle Saab, Thierry Cloitre, Frédérique Scamps, Csilla Gergely, and Marta Martin "Changes induced by peripheral nerve injury in the morphology and nanomechanics of sensory neurons", Proc. SPIE 8797, Advanced Microscopy Techniques III, 879704 (17 June 2013);

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