CARS and non-linear microscopy imaging of brain tumors

Roberta Galli; Ortrud Uckermann; Sandra Tamosaityte; Kathrin Geiger; Gabriele Schackert; Gerald Steiner; Edmund Koch; Matthias Kirsch

doi:10.1117/12.2031729

17 June 2013 CARS and non-linear microscopy imaging of brain tumors

Roberta Galli, Ortrud Uckermann, Sandra Tamosaityte, Kathrin Geiger, Gabriele Schackert, Gerald Steiner, Edmund Koch, Matthias Kirsch

Author Affiliations +

Proceedings Volume 8797, Advanced Microscopy Techniques III; 87970E (2013) https://doi.org/10.1117/12.2031729
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

Nonlinear optical microscopy offers a series of techniques that have the potential to be applied in vivo, for intraoperative identification of tumor border and in situ pathology. By addressing the different content of lipids that characterize the tumors with respect to the normal brain tissue, CARS microscopy enables to discern primary and secondary brain tumors from healthy tissue. A study performed in mouse models shows that the reduction of the CARS signal is a reliable quantity to identify brain tumors, irrespective from the tumor type. Moreover it enables to identify tumor borders and infiltrations at a cellular resolution. Integration of CARS with autogenous TPEF and SHG adds morphological and compositional details about the tissue. Examples of multimodal CARS imaging of different human tumor biopsies demonstrate the ability of the technique to retrieve information useful for histopathological diagnosis.

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Roberta Galli, Ortrud Uckermann, Sandra Tamosaityte, Kathrin Geiger, Gabriele Schackert, Gerald Steiner, Edmund Koch, and Matthias Kirsch "CARS and non-linear microscopy imaging of brain tumors", Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970E (17 June 2013); https://doi.org/10.1117/12.2031729

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