18 June 2013 Near infrared FRET using wide-field fluorescence lifetime imaging in live animals
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One of the challenges in anti-cancer drug delivery systems is to quantitatively discriminate non-specific receptorindependent tumor accumulation from receptor-mediated uptake into the tumor cells. To overcome this challenge, we develop a new near infrared fluorescence resonance energy transfer fluorescence lifetime imaging (NIR FRET FLIM) technique with wide-field illumination strategies to validate and characterize cellular uptake in both cancer cells and normal cells with different donor-acceptor ratios in vitro and in vivo. Our results demonstrate that NIR FRET FLIM can quantitatively distinguish receptor-bound from unbound donor in live animals with high sensitivity and high accuracy. Thus, it has a great potential for the quantitative detection of targeted delivery systems for diagnostic and therapeutic use.
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Lingling Zhao, Ken Abe, Margarida Barroso, and Xavier Intes "Near infrared FRET using wide-field fluorescence lifetime imaging in live animals", Proc. SPIE 8801, Novel Biophotonic Techniques and Applications II, 88010A (18 June 2013); doi: 10.1117/12.2033588; https://doi.org/10.1117/12.2033588

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