12 September 2013 Measurement of the signal from a single DNA molecule trapped by a nanoplasmonic structure
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Abstract
Optical tweezers use focused laser to trap microobjects suspended in the medium to the focal point. They are becoming an indispensable tool in microbiology because of its ability to trap tiny biological particles so that single particle analysis is possible. However, it is still very difficult to trap particles such as DNA molecules that are smaller than the diffraction limit. Although trapping of those is possible by increasing the laser power inversely proportional to the cube of the particle diameter, such high power can cause permanent thermal damages. One of the current solutions to this problem is to intensify the local field by the use of the near-field enhancement coming from nanoplasmonic structures illuminated with lasers. Such solution allows one to use low powered laser and still be able to trap them. In this paper, we present the trapping of a single DNA molecule by the use of the strong field enhancement due to a sub-micrometer sized hole drilled on a gold plate by an e-beam milling process and the trapping is verified by the measurement of the scattering signal that comes from the trapped DNA.
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Jung-Dae Kim, Waleed Muhammad, Yong-Gu Lee, "Measurement of the signal from a single DNA molecule trapped by a nanoplasmonic structure", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88100W (12 September 2013); doi: 10.1117/12.2022774; https://doi.org/10.1117/12.2022774
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