12 September 2013 Optical manipulation of vesicles for optofluidic applications
Author Affiliations +
In this report, we review our recent results in the optical micromanipulation of vesicles. Traditionally, vesicle manipulation has been possible by employing photon momentum and optical trapping, giving rise to unique observations of vesicle shape changes and soft matter mechanics. Contrary to these attempts, we employ photon energy rather than momentum, by sensitizing vesicles with an oxidizing moiety. The later converts incident photons to reactive oxygen species, which in turn attack and compromise the stability of the vesicle membrane. Both coherent and incoherent radiation was employed. Polymersome re-organization into smaller diameter vesicles was possible by focusing the excitation beam in the vicinity of the polymersomes. Extended vesicle illumination with a collimated beam lead to their complete destabilization and micelle formation. Single particle analysis revealed that payload release takes place within seconds of illumination in an explosive burst. We will discuss the destabilization and payload release kinetics, as revealed by high resolution microscopy at the single particle level, as well as potential applications in single cell biomodulation.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
A. E. Vasdekis, A. E. Vasdekis, E. A. Scott, E. A. Scott, C. P. O'Neil, C. P. O'Neil, D. Psaltis, D. Psaltis, J. A. Hubbell, J. A. Hubbell, "Optical manipulation of vesicles for optofluidic applications", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88101R (12 September 2013); doi: 10.1117/12.2026583; https://doi.org/10.1117/12.2026583


Probing multiscale mechanics of collagen with optical tweezers
Proceedings of SPIE (September 11 2013)
Motion of matter induced by light fueled molecular machines
Proceedings of SPIE (September 13 2018)
Optoelectronic integrated tweezers
Proceedings of SPIE (October 17 2004)
Particle dynamics in optical lattices
Proceedings of SPIE (August 19 2009)
Compact laser tweezers
Proceedings of SPIE (April 11 2007)

Back to Top