12 September 2013 Heat in optical tweezers
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Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.
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B. del Rosal, B. del Rosal, P. Haro-González, P. Haro-González, W. T. Ramsay, W. T. Ramsay, L. M. Maestro, L. M. Maestro, K. Santacruz-Gómez, K. Santacruz-Gómez, M. C. Iglesias-de la Cruz, M. C. Iglesias-de la Cruz, F. Sanz-Rodríguez, F. Sanz-Rodríguez, J. Y. Chooi, J. Y. Chooi, P. Rodríguez-Sevilla, P. Rodríguez-Sevilla, D. Choudhury, D. Choudhury, A. K. Kar, A. K. Kar, J. García Solé, J. García Solé, L. Paterson, L. Paterson, D. Jaque, D. Jaque, "Heat in optical tweezers", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88102A (12 September 2013); doi: 10.1117/12.2027750; https://doi.org/10.1117/12.2027750

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