11 September 2013 Novel optical strategies for biodetection
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Although bio-detection strategies have significantly evolved in the past decade, they still suffer from many disadvantages. For one, current approaches still require confirmation of pathogen viability by culture, which is the ‘gold-standard’ method, and can take several days to result. Second, current methods typically target protein and nucleic acid signatures and cannot be applied to other biochemical categories of biomarkers (e.g.; lipidated sugars). Lipidated sugars (e.g.; lipopolysaccharide, lipoarabinomannan) are bacterial virulence factors that are significant to pathogenicity. Herein, we present two different optical strategies for biodetection to address these two limitations. We have exploited bacterial iron sequestration mechanisms to develop a simple, specific assay for the selective detection of viable bacteria, without the need for culture. We are currently working on the use of this technology for the differential detection of two different bacteria, using siderophores. Second, we have developed a novel strategy termed ‘membrane insertion’ for the detection of amphiphilic biomarkers (e.g. lipidated glycans) that cannot be detected by conventional approaches. We have extended this technology to the detection of small molecule amphiphilic virulence factors, such as phenolic glycolipid-1 from leprosy, which could not be directly detected before. Together, these strategies address two critical limitations in current biodetection approaches. We are currently working on the optimization of these methods, and their extension to real-world clinical samples.
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Rama Murthy Sakamuri, Rama Murthy Sakamuri, Mark S. Wolfenden, Mark S. Wolfenden, Aaron S. Anderson, Aaron S. Anderson, Basil I. Swanson, Basil I. Swanson, Jurgen S. Schmidt, Jurgen S. Schmidt, Harshini Mukundan, Harshini Mukundan, "Novel optical strategies for biodetection", Proc. SPIE 8812, Biosensing and Nanomedicine VI, 881209 (11 September 2013); doi: 10.1117/12.2029605; https://doi.org/10.1117/12.2029605

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