11 September 2013 Use of novel silver nanoparticles with Hyaluronan as potential biological labels for determining the quality of embryos development
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In reproductive medicine it is important to determine the quality of embryo development without interference in their function and viability. The surface plasmon resonance of silver nanoparticles makes them promising candidates for optical sensing, molecular labeling and imaging applications. Furthermore unique optical properties of silver nanoparticles provide an opportunity to use them as real time analytic tools in living state especially for observation of dynamic processes in gametes and embryos. The main aim of the study was to investigate the physicochemical properties and biological activities of novel silver nanoparticles with prospect of their use for the determining the quality of embryo development. For this purpose, we investigated the optical properties of new silver nanoparticles in biological mediums during cultivation and their influence on rabbit’s embryos development in vitro. The physicochemical and biological properties of novel silver nanoparticles were compared with silver nanoparticles identical in size and shapes but with different chemical surfaces modifications by polyvinylpyrrolidone and bovine serum albumin. The results suggest that silver nanoparticles with hyaluronic acid were disintegrated with the formation of new complexes with proteins in biological mediums. This property with strong optical surface plasmon resonance of novel silver nanoparticles with hyaluronan makes them promising candidates in diagnostic area and gives reasons to explore them as biomarkers of target molecules. Nevertheless novel silver nanoparticles with hyaluronan at the concentrations of 0.1-1 μg/ml have no toxic effect on rabbit’s embryos development and can be successfully applied in reproductive medicine.
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Vasyl J. Syrvatka, Yurij I. Slyvchuk, Ivan I. Rozgoni, Ivan I. Hevkan, Oleksandr I. Bilyi, Oleksandr S. Osypchuk, Aza B. Zyuzyun, "Use of novel silver nanoparticles with Hyaluronan as potential biological labels for determining the quality of embryos development", Proc. SPIE 8812, Biosensing and Nanomedicine VI, 88120I (11 September 2013); doi: 10.1117/12.2023646; https://doi.org/10.1117/12.2023646

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