11 September 2013 The interaction of the carbon nanoparticles with human cell plasma membrane
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The study of carbon nanostructures is a highly topical branch of bionanotechnology because of their potential application in biomedicine. Carbon nanotubes (CNTs) are known for their ability to kill tumor cells causing hyperthermia shock and can be used in photothermal therapy respectively. Also chemically modified CNTs can be used for drug delivery. The needle-like shape of CNTs allows them to penetrate into the cell plasma membrane without killing the cell. C60 fullerenes are regarded as valuable nanocarriers for different hydrophobic molecules as well as potential antiviral agents or photosensitizers. In our previous studies we have demonstrated that all types of carbon nanoparticles cause externalization of phosphatidylserine (PS) from the inner to the outer layer of the cell membrane in the small local patches (points of contact), leaving the other parts of plasma membrane PS-negative. In the current work there were studied the interactions of pristine C60 fullerenes and different types of CNTs with human blood cells (erythrocytes and Jurkat T-cells). We have shown, that carbon nanoparticles do not have any hemolytic effects, if judged by the dynamics of acidic hemolysis, although they are capable of permeabilizating the cells and facilitating the internalization of propidium iodide into the nuclei.
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M. Overchuk, M. Overchuk, S. Prylutska, S. Prylutska, Rostyslav Bilyy, Rostyslav Bilyy, Yu. Prylutsky, Yu. Prylutsky, U. Ritter, U. Ritter, "The interaction of the carbon nanoparticles with human cell plasma membrane", Proc. SPIE 8812, Biosensing and Nanomedicine VI, 88120X (11 September 2013); doi: 10.1117/12.2023271; https://doi.org/10.1117/12.2023271

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