20 September 2013 Image resolution in optical nanoscopy
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Super-resolution microscopy often employs asynchronous localizations of many single fluorescent emitters achieving resolution below the diffraction limit. This family of techniques typically uses statistical switching of emitters between dark and bright fluorescent states. Here we investigate how imaging repeated activations cycles of the same emitter influences the achieved image resolution. Furthermore, we ask the questions how long such a typical bright emitting state should be and is there an optimal number of switching events if the measurement time is fixed. We find that longer measurement times and hereby imaging more activation cycles is always beneficial for the attained image resolution. In the case of a fixed measurement time it turns out that there is a trade-off between the number of cycles and the product of localization density and uncertainty.
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Robert P. J. Nieuwenhuizen, Robert P. J. Nieuwenhuizen, Sjoerd Stallinga, Sjoerd Stallinga, Bernd Rieger, Bernd Rieger, "Image resolution in optical nanoscopy", Proc. SPIE 8815, Nanoimaging and Nanospectroscopy, 881508 (20 September 2013); doi: 10.1117/12.2025352; https://doi.org/10.1117/12.2025352

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