Cryogenic localization of single molecules with angstrom precision

Siegfried Weisenburger; Bo Jing; Alois Renn; Vahid Sandoghdar

doi:10.1117/12.2025373

20 September 2013 Cryogenic localization of single molecules with angstrom precision

Siegfried Weisenburger, Bo Jing, Alois Renn, Vahid Sandoghdar

Proceedings Volume 8815, Nanoimaging and Nanospectroscopy; 88150D (2013) https://doi.org/10.1117/12.2025373
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

The precision in localizing a molecule is ultimately determined by the number of detected photons, which is in turn limited by photobleaching. Currently, fluorophores can be routinely localized to a few tens of nanometers at room temperature. In this work we demonstrate localization precision better than 3 Angstrom by substantial improvement of the molecular photostability at cryogenic temperatures. We discuss the challenges, solutions and promise of our methodology for high-performance co-localization and super-resolution microscopy.

Citation Download Citation

Siegfried Weisenburger, Bo Jing, Alois Renn, and Vahid Sandoghdar "Cryogenic localization of single molecules with angstrom precision", Proc. SPIE 8815, Nanoimaging and Nanospectroscopy, 88150D (20 September 2013); https://doi.org/10.1117/12.2025373

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