Second harmonic generation from tyrosine containing peptides

M. N. Nasir; E. Bergmann; E. Benichou; I. Russier-Antoine; N. Lascoux; Ch. Jonin; F. Besson; P. F. Brevet

doi:10.1117/12.2026866

1 October 2013 Second harmonic generation from tyrosine containing peptides

M. N. Nasir, E. Bergmann, E. Benichou, I. Russier-Antoine, N. Lascoux, Ch. Jonin, F. Besson, P. F. Brevet

Proceedings Volume 8817, Nanobiosystems: Processing, Characterization, and Applications VI; 88170M (2013) https://doi.org/10.1117/12.2026866
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

The Second Harmonic Generation (SHG) response from Tyrosine-containing peptides at the air-water interface is presented. First, the quadratic hyperpolarizability of the aromatic amino acid Tyrosine obtained by Hyper Rayleigh Scattering is reported, demonstrating its potentiality as an endogenous molecular probe for SHG studies. Then, the single Tyrosine antimicrobial peptide Mycosubtilin is monitored at the air-water interface and compared to another peptide, Surfactin, lacking a Tyrosine residue. Adsorption kinetics and polarization analysis of the SHG intensity for the peptide monolayers clearly demonstrate that the SHG response from Mycosubtilin arises from Tyrosine. Besides, it confirms that indeed Tyrosine can be targeted as an endogenous molecular probe.

Citation Download Citation

M. N. Nasir, E. Bergmann, E. Benichou, I. Russier-Antoine, N. Lascoux, Ch. Jonin, F. Besson, and P. F. Brevet "Second harmonic generation from tyrosine containing peptides", Proc. SPIE 8817, Nanobiosystems: Processing, Characterization, and Applications VI, 88170M (1 October 2013); https://doi.org/10.1117/12.2026866

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