Motion compensation of optical mapping signals from isolated beating rat hearts

B. Stender; F. Ernst; B. Wang; Z. X. Zhang; A. Schlaefer

doi:10.1117/12.2024847

26 September 2013 Motion compensation of optical mapping signals from isolated beating rat hearts

B. Stender, F. Ernst, B. Wang, Z. X. Zhang, A. Schlaefer

Proceedings Volume 8856, Applications of Digital Image Processing XXXVI; 88561C (2013) https://doi.org/10.1117/12.2024847
Event: SPIE Optical Engineering + Applications, 2013, San Diego, California, United States

Abstract

Optical mapping is a well established technique for recording monophasic action potential traces on the epicardial surface of isolated hearts. This measuring technique offers a high spatial resolution but it is sensitive towards myocardial motion. Motion artifacts occur because the mapping between a certain tissue portion sending out fluorescent light and a pixel of the photo detector changes over time. So far this problem has been addressed by suppressing the motion or ratiometric imaging. We developed a different approach to compensate the motion artifacts based on image registration. We could demonstrate how an image deformation field temporally changing with the heart motion could be determined. Using these deformation field time series for image transformation motion signals could be generated for each image pixel which were then successfully applied to remove baseline shift and compensate motion artifacts potentially leading to errors within maps of the first arrival time. The investigation was based on five different rat hearts stained with Di-4-ANEPPS.

Citation Download Citation

B. Stender, F. Ernst, B. Wang, Z. X. Zhang, and A. Schlaefer "Motion compensation of optical mapping signals from isolated beating rat hearts", Proc. SPIE 8856, Applications of Digital Image Processing XXXVI, 88561C (26 September 2013); https://doi.org/10.1117/12.2024847

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available