Directional immobilization of antibody in a SPR sensor using EDC-activated protein A

Yeon Kyung Lee; Jeong-Ok Lim; Young-Soo Sohn

doi:10.1117/12.2017746

Translator Disclaimer

20 May 2013 Directional immobilization of antibody in a SPR sensor using EDC-activated protein A

Yeon Kyung Lee, Jeong-Ok Lim, Young-Soo Sohn

Author Affiliations +

Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 887904 (2013) https://doi.org/10.1117/12.2017746
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

The EDC-activated protein A has been utilized to directionally immobilize anti-IgG in a miniaturized SPR sensor to enhance IgG detection capability. The SPR sensor chips modified by a self-assembled monolayer (SAM), protein A and the EDC-activated protein A as the linkage layer were compared by the SPR sensor. The SAM was formed on the Au (gold) surface sensor chip by immersing it in the SAM solution. The protein A was formed by injecting their solution to the Au chip. Thirdly, for the EDC-activated protein A, chemical procedure was carried out for the reactable surface of the Au chip. Anti-IgG, bovine serum albumin (BSA) and IgG (50 ng/ml, 100 ng/ml, 150 ng/ml) had been sequently injected into the SPR sensor. In results, the signal of the anti-IgG immobilized by the SAM was the largest increment among three linkage layers. However, the SPR sensor chip modified by EDC-activated protein A showed the highest sensitivity to IgG. From these results, we concluded that the SPR sensor using the EDC-activated protein A can be used to detect biomolecules with trace level concentration for early diagnosis of disease.

Citation Download Citation

Yeon Kyung Lee, Jeong-Ok Lim, and Young-Soo Sohn "Directional immobilization of antibody in a SPR sensor using EDC-activated protein A", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887904 (20 May 2013); https://doi.org/10.1117/12.2017746

ACCESS THE FULL ARTICLE