Real-time detection of neurite outgrowth using microfluidic device

Samhwan Kim; Jongmoon Jang; Hongsoo Choi; Cheil Moon

doi:10.1117/12.2018667

20 May 2013 Real-time detection of neurite outgrowth using microfluidic device

Samhwan Kim, Jongmoon Jang, Hongsoo Choi, Cheil Moon

Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 887906 (2013) https://doi.org/10.1117/12.2018667
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.

Citation Download Citation

Samhwan Kim, Jongmoon Jang, Hongsoo Choi, and Cheil Moon "Real-time detection of neurite outgrowth using microfluidic device", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887906 (20 May 2013); https://doi.org/10.1117/12.2018667

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available