Insights into esophagus tissue architecture using two-photon confocal microscopy

Nenrong Liu; Yue Wang; Shangyuan Feng; Rong Chen

doi:10.1117/12.2035041

30 August 2013 Insights into esophagus tissue architecture using two-photon confocal microscopy

Nenrong Liu, Yue Wang, Shangyuan Feng, Rong Chen

Proceedings Volume 8910, International Symposium on Photoelectronic Detection and Imaging 2013: Imaging Spectrometer Technologies and Applications; 891022 (2013) https://doi.org/10.1117/12.2035041
Event: ISPDI 2013 - Fifth International Symposium on Photoelectronic Detection and Imaging, 2013, Beijing, China

Abstract

In this paper, microstructures of human esophageal mucosa were evaluated using the two-photon laser scanning confocal microscopy (TPLSCM), based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG). The distribution of epithelial cells, muscle fibers of muscularis mucosae has been distinctly obtained. Furthermore, esophageal submucosa characteristics with cancer cells invading into were detected. The variation of collagen, elastin and cancer cells is very relevant to the pathology in esophagus, especially early esophageal cancer. Our experimental results indicate that the MPM technique has the much more advantages for label-free imaging, and has the potential application in vivo in the clinical diagnosis and monitoring of early esophageal cancer.

Citation Download Citation

Nenrong Liu, Yue Wang, Shangyuan Feng, and Rong Chen "Insights into esophagus tissue architecture using two-photon confocal microscopy ", Proc. SPIE 8910, International Symposium on Photoelectronic Detection and Imaging 2013: Imaging Spectrometer Technologies and Applications, 891022 (30 August 2013); https://doi.org/10.1117/12.2035041

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