Poyang Lake Basin is the biggest freshwater lake in China and a significant wetland of the world. The study of the land use changes on there is a great significance for regional sustainable development. In this paper, using the RS image as the main data source, the study area was divided into six land use types. With the acquired land use data of three periods, the spatio-temporal dynamic variation characteristics were analyzed with the area changes and land use dynamic index (LUDI). The analysis shows that the largest land use type is woodland, followed by cultivated land and grassland, and area of the rest types all account for less than 10%. Through the analysis of the area transfer matrix of LUCC, it shows that woodland and construction land increased in each period while cultivated land reduced. Unused land increased a lot during 1990 to 2000 before decreased dramatically during 2000 to 2008, grassland and water experienced a significant increase after an obvious decline and came out to increase finally. The analysis of LUDI indicates that construction land changed the most quickly, followed by unused land and cultivated land, yet the other land use types changed slowly.