Laser measurements of bacterial endospore destruction from shock waves

Petros P. Lappas; A. Daniel McCartt; Sean D. Gates; Jay B. Jeffries; Ronald K. Hanson

doi:10.1117/12.2033936

7 December 2013 Laser measurements of bacterial endospore destruction from shock waves

Petros P. Lappas, A. Daniel McCartt, Sean D. Gates, Jay B. Jeffries, Ronald K. Hanson

Proceedings Volume 8923, Micro/Nano Materials, Devices, and Systems; 89231C (2013) https://doi.org/10.1117/12.2033936
Event: SPIE Micro+Nano Materials, Devices, and Applications, 2013, Melbourne, Victoria, Australia

Abstract

The effects of shock waves on bioaerosols containing endospores were measured by combined laser absorption and scattering. Experiments were conducted in the Stanford aerosol shock tube for post-shock temperatures ranging from 400 K to 1100 K. Laser intensity measurements through the test section of the shock tube at wavelengths of 266 and 665 nm provided real-time monitoring of the morphological changes (includes changes in shape, structure and optical properties) in the endospores. Scatter of the visible light measured the integrity of endospore structure, while absorption of the UV light provided a measure of biochemicals released when endospores ruptured. For post-shock temperatures above 750 K the structural breakdown of Bacillus atrophaeus (BA) endospores was observed. A simple theoretical model using laser extinction is presented for determining the fraction of endospores that are ruptured by the shock waves. In addition, mechanisms of endospore mortality preceding their disintegration due to shock waves are discussed.

Citation Download Citation

Petros P. Lappas, A. Daniel McCartt, Sean D. Gates, Jay B. Jeffries, and Ronald K. Hanson "Laser measurements of bacterial endospore destruction from shock waves", Proc. SPIE 8923, Micro/Nano Materials, Devices, and Systems, 89231C (7 December 2013); https://doi.org/10.1117/12.2033936

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available