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4 March 2014Sensing vascularization of ex-vivo produced oral mucosal equivalent (EVPOME) skin grafts in nude mice using optical spectroscopy
Repair of soft tissue defects of the lips as seen in complex maxillofacial injuries, requires pre-vascularized multi-tissue
composite grafts. Protocols for fabrication of human ex-vivo produced oral mucosal equivalents (EVPOME) composed
of epithelial cells and a dermal equivalent are available to create prelaminated flaps for grafting in patients. However, invivo
assessment of neovascularization of the buried prelaminated flaps remains clinically challenging. Here, we use
diffuse reflectance spectroscopy (DRS) and diffuse correlation spectroscopy (DCS) to non-invasively quantify
longitudinal changes in the vessel density and blood-flow within EVPOME grafts implanted in the backs of SCID mice
and subsequently to determine the utility of these optical techniques for assessing vascularization of implanted grafts. 20
animals were implanted with EVPOME grafts (1x1x0.05 cm3) in their backs. DRS and DCS measurements were
obtained from each animal both atop the graft site and far away from the graft site, at one week post-implantation, each
week, for four consecutive weeks. DRS spectra were analyzed using an inverse Monte Carlo model to extract tissue
absorption and scattering coefficients, which were then used to extract blood flow information by fitting the
experimental DCS traces. There were clear differences in the mean optical parameters (averaged across all mice) at the
graft site vs. the off-site measurements. Both the total hemoglobin concentration (from DRS) and the relative blood flow
(from DCS) peaked at week 3 at the graft site and declined to the off-site values by week 4. The optical parameters
remained relatively constant throughout 4 weeks for the off-site measurements.
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Karthik Vishwanath, Rajan Gurjar, Shiuhyang Kuo, Anthony Fasi, Roderick Kim, Suzannah Riccardi, Stephen E. Feinberg, David E. Wolf, "Sensing vascularization of ex-vivo produced oral mucosal equivalent (EVPOME) skin grafts in nude mice using optical spectroscopy," Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89260I (4 March 2014); https://doi.org/10.1117/12.2037085