Actinic cheilitis (AC) is considered a potentially malignant disorder that mainly affects the
lower lip, and it is caused by prolonged sun exposure. Clinical diagnosis relies on visual
inspection by a trained clinician, when suspected of dysplasia changes, a biopsy is required.
The heteregenous characteristics of the AC, makes the choice of the biopsy site a difficult task.
Fluorescence detection has been presented as a useful tool to to detect biochemical and
morphological tissue features related to cancer diagnosis, but still its effectiveness to
discriminate premalignant lesion is not completely defined. In this clinical study, 57 AC patients
were investigated using widefield fluorescence imaging (WFI) to evaluate the efficacy of this
technique as an auxiliary tool to biopsy site location. A handheld fluorescence system based on
400-450 nm LED illumination Distinct trained clinicians evaluate the patient either with the
conventional examination or the WFI, and were blinded to the other evaluation. A biopsy site
was chosen based on the clinical examination, and another site was chosen using the
fluorescence visualization. A total of 114 punch biopsies were performed, and 93% of the
tissue samples presented epithelial dysplasia. The majority of the sites that presented
moderate or severe dysplasia were sites chosen by WFI, showing its efficiency to improve the
diagnosis of AC.