Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic analysis of regenerated bone

Carolina Benetti; Sergei G. Kazarain; Marco A. V. Alves; Alberto Blay; Luciana Correa; Denise M. Zezell

doi:10.1117/12.2039915

4 March 2014 Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic analysis of regenerated bone

Carolina Benetti, Sergei G. Kazarain, Marco A. V. Alves, Alberto Blay, Luciana Correa, Denise M. Zezell

Author Affiliations +

Proceedings Volume 8926, Photonic Therapeutics and Diagnostics X; 892641 (2014) https://doi.org/10.1117/12.2039915
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The cutting of bone is routinely required in medical procedures, especially in dental applications. In such cases, bone regeneration and new bone quality can determine the success of the treatment. This study investigated the main spectral differences of undamaged and healed bone using the ATR-FTIR spectroscopy technique. Three rabbits were submitted to a surgical procedure; a small piece of bone (3x3 mm²) was removed from both sides of their jaws using a high speed drill. After 15 days, the rabbits were euthanized and the jaws were removed. A bone slice was cut from each side of the jaw containing regions of undamaged and newly formed bone, resulting in six samples which were polished for spectroscopic comparison. The samples were analyzed by FTIR spectroscopy using a diamond ATR accessory. Spectral characteristics were compared and particular attention was paid to the proportion of phosphate to amide I bands and the width of the phosphate band. The results show that the ratio of phosphate to amide I is smaller in new bone tissue than in the undamaged bone, indicating a higher organic content in the newly formed bone. The analysis of the width of the phosphate band suggests a crystallinity difference between both tissues, since the width was higher in the new bone than in the natural bone. These results suggest that the differences observed in bone aging processes by FTIR spectroscopic can be applied to the study of healing processes.

Citation Download Citation

Carolina Benetti, Sergei G. Kazarain, Marco A. V. Alves, Alberto Blay, Luciana Correa, and Denise M. Zezell "Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic analysis of regenerated bone", Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 892641 (4 March 2014); https://doi.org/10.1117/12.2039915

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