4 March 2014 Multi-spectral scanning fiber endoscope with concurrent autofluorescence mitigation for enhanced target-to-background ratio imaging
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Abstract
We developed a multispectral fluorescence-reflectance scanning fiber endoscope (SFE) for wide-field molecular imaging of fluorescence-labeled molecular probes. Concurrent multi-channels imaging with the wide-field SFE also allows for real-time mitigation of background autofluorescence (AF) signal, especially when the FDA approved fluorescein is used as the target fluorophore. In the current study, we demonstrated a real-time AF mitigation algorithm on a tissue phantom which featured molecular probe targeted cells of high grade dysplasia on a substrate containing AF species. The targetto- background ratio was enhanced by over an order of magnitude when applying the real-time AF mitigation algorithm. By minimizing the background signal, multispectral fluorescence imaging can provide sufficient image contrast and quantitative target information for detecting small pre-cancerous lesions in vivo.
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Chenying Yang, Vivian W. Hou, Leonard Y. Nelson, Richard S. Johnston, C. David Melville, Eric J. Seibel, "Multi-spectral scanning fiber endoscope with concurrent autofluorescence mitigation for enhanced target-to-background ratio imaging", Proc. SPIE 8927, Endoscopic Microscopy IX; and Optical Techniques in Pulmonary Medicine, 89270I (4 March 2014); doi: 10.1117/12.2038553; https://doi.org/10.1117/12.2038553
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