5 March 2014 Photothermal therapy of human glioma spheroids with gold-silica nanoshells and gold nanorods: a comparative study
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Abstract
The efficacy of gold-silica nanoshells (AuNS) and gold nanorods (AuNR) for photothermal therapy was investigated in an in vitro system consisting of hybrid murine macrophage/human glioma spheroids. Macrophages were used as delivery vectors for the nanoparticles. Hybrid spheroids were formed via centrifugation of human glioma cells and nanoparticleloaded macrophages. Forty-eight hours post-centrifugation, the resultant 400 μm dia. spheroids were exposed to 808 nm laser light for 10 min. at irradiances ranging from 2 - 28 W cm-2. Treatment efficacy was evaluated from spheroid growth kinetics over a 14-day period. AuNS were shown to have greater efficacy compared to AuNR. For example, hybrid spheroids consisting of a 5:1 ratio of glioma cells to AuNS-loaded macrophages exhibited significant growth inhibition when subjected to irradiances of 7 W cm-2. In contrast, no growth inhibition was observed for the AuNR-macrophage hybrid spheroids, even at the highest irradiance investigated (28 W cm-2). Growth inhibition was observed at 28 W cm-2 when the nanorod concentration was increased, i.e., by forming hybrid spheroids with a 2:1 ratio of glioma cells to macrophages.
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Suyog Chhetri, Henry Hirschberg, Steen J. Madsen, "Photothermal therapy of human glioma spheroids with gold-silica nanoshells and gold nanorods: a comparative study", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89280U (5 March 2014); doi: 10.1117/12.2038233; https://doi.org/10.1117/12.2038233
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