5 March 2014 Imaging of rat brain using short graded-index multimode fiber
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Abstract
Clinically it is important to image structures of brain at deeper areas with low invasions, for example, the pathological information is not obtained enough from the white matter. Preliminarily we have measured transmission images of rat brain using the short graded-index multimode fiber (SMMF) with the diameter of 140μm and length of 5mm. SMMF (core diameter, 100μm) was cut using a fiber cleaver and was fixed in a jig. Fiber lengths inside and outside jig were 3mm and 2mm, respectively. The jig was attached at the 20x objective lens. The conventional optical microscope was used to measure images. In basic characteristics, it was confirmed that the imaging conditions almost corresponded to calculations with the ray-transfer matrix and the spatial resolution was evaluated at about 4.4μm by measuring the test pattern. After euthanasia the rat parietal brain was excised with thickness around 1.5mm and was set on the slide glass. The tissue was illuminated through the slide glass by the bundle fiber with Halogen lamp. The tip of SMMF was inserted into the tissue by lifting the sample stage. The transmission image at each depth from 0.1mm to 1.53mm was measured. Around the depth of 1.45mm, granular structures with sizes of 4-5μm were recognized and corresponded to images by HE stained tissue. Total measurement time was within 2 hours. The feasibilities to image the depth of 5 mm with SMMF have been shown.
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Manabu Sato, Takahiro Kanno, Syoutarou Ishihara, Hiroshi Suto, Toshihiro Takahashi, Reiko Kurotani, Hiroyuki Abe, Izumi Nishidate, "Imaging of rat brain using short graded-index multimode fiber", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89281D (5 March 2014); doi: 10.1117/12.2036986; https://doi.org/10.1117/12.2036986
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