Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice

Jaepyeong Cha; Martin Paukert; Dwight E. Bergles; Jin U. Kang

doi:10.1117/12.2038265

20 March 2014 Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice

Jaepyeong Cha, Martin Paukert, Dwight E. Bergles, Jin U. Kang

Proceedings Volume 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 89282N (2014) https://doi.org/10.1117/12.2038265
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Fiber-optic based optical imaging is an emerging technique for studying brain activity in live animals. Here, we introduce a novel fluorescence fiber-optic microendoscopy approach to minimal invasively detect neural activities in a live mouse brain . The system uses a flexible endoscopic probe composed of a multi-core coherent fiber-bundle terminated with an approximately 1500-micron working distance objective lens. The fiber-optic neural interface is mounted on a 4-mm² cranial window enabling visualization of glial calcium transients from the same brain region for weeks. We evaluated the system performance through in vivo imaging of GCaMP3 fluorescence in transgenic headrestrained mice during locomotion.

Citation Download Citation

Jaepyeong Cha, Martin Paukert, Dwight E. Bergles, and Jin U. Kang "Fiber optic fluorescence microscopy for functional brain imaging in awake, mobile mice", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89282N (20 March 2014); https://doi.org/10.1117/12.2038265

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