Structure and chemical composition of the dentin-enamel junction analyzed by Confocal Raman Microscopy

A. Desoutter; H. Salehi; A. Slimani; P. Marquet; B. Jacquot; H. Tassery; F. J. G. Cuisinier

doi:10.1117/12.2039826

18 February 2014 Structure and chemical composition of the dentin-enamel junction analyzed by Confocal Raman Microscopy

A. Desoutter, H. Salehi, A. Slimani, P. Marquet, B. Jacquot, H. Tassery, F. J. G. Cuisinier

Proceedings Volume 8929, Lasers in Dentistry XX; 892907 (2014) https://doi.org/10.1117/12.2039826
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The structure and chemical composition of the human dentin-enamel junction (DEJ) was studied using confocal Raman microscopy - a chemical imaging technique. Slices of non-fixed, sound teeth were prepared with an Isomet diamond saw and scanned with Witec Alpha300R system. The combination of different characteristics peaks of phosphate, carbonate and organic matrix (respectively 960, 1072 and 1545 cm^-1), generates images representing the chemical composition of the DEJ area. Images are also calculated using peak ratios enabling precise determination of the chemical composition across the DEJ. Then, with two characterized peaks, different pictures are calculated to show the ratio of two components. The images of the spatial distribution of mineral phosphate (960cm^-1) to organic matrix (1545 cm^-1) ratios, mineral carbonates (1072cm^-1) to mineral phosphate ratios; and mineral carbonates to organic matrix ratios were reconstructed. Cross sectional and calculated graphic profile show the variations of the different chemical component ratios through the enamel and the dentin. Phosphate to organic ratio shows an accumulation of organic material under the enamel surface. The cross sectional profile of these pictures shows a high phosphate content compared to enamel in the vicinity of the DEJ. The Confocal Raman imaging technique can be used to further provide full chemical imaging of tooth, particularly of the whole DEJ and to study enamel and dentin decay.

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A. Desoutter, H. Salehi, A. Slimani, P. Marquet, B. Jacquot, H. Tassery, and F. J. G. Cuisinier "Structure and chemical composition of the dentin-enamel junction analyzed by Confocal Raman Microscopy", Proc. SPIE 8929, Lasers in Dentistry XX, 892907 (18 February 2014); https://doi.org/10.1117/12.2039826

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