You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.
14 April 2014Increased efficacy of photodynamic therapy via sequential targeting
Photokilling depends on the generation of death signals after photosensitized cells are irradiated. A variety of intracellular
organelles can be targeted for photodamage, often with a high degree of specificity. We have discovered that a low level
of photodamage directed against lysosomes can sensitize both a murine hepatoma cell line (in 2D culture) and an
inflammatory breast cancer line of human origin (in a 3D model) to subsequent photodamage directed at mitochondria.
Additional studies were carried out with hepatoma cells to explore possible mechanisms. The phototoxic effect of the
‘sequential targeting’ approach was associated with an increased apoptotic response. The low level of lysosomal
photodamage did not lead to any detectable migration of Fe++ from lysosomes to mitochondria or increased reactive oxygen species (ROS) formation after subsequent mitochondrial photodamage. Instead, there appears to be a signal generated that
can amplify the pro-apoptotic effect of subsequent mitochondrial photodamage.
David Kessel,Neha Aggarwal, andBonnie F. Sloane
"Increased efficacy of photodynamic therapy via sequential targeting", Proc. SPIE 8931, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII, 893102 (14 April 2014); https://doi.org/10.1117/12.2042421
The alert did not successfully save. Please try again later.
David Kessel, Neha Aggarwal, Bonnie F. Sloane, "Increased efficacy of photodynamic therapy via sequential targeting," Proc. SPIE 8931, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII, 893102 (14 April 2014); https://doi.org/10.1117/12.2042421