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14 April 2014Evaluation of the Photodynamic Therapy effect using a tumor model in Chorioallantoic Membrane with Melanoma cells
Photodynamic Therapy (PDT) is a type of cancer treatment that is based on the interaction of light (with specific
wavelength), a photosensitizing agent and molecular oxygen. The photosensitizer (PS) is activated by light and reacts
with oxygen resulting in the production of singlet oxygen that is highly reactive and responsible for the cell death. The
Chick Chorioallantoic Membrane (CAM) model is a transparent membrane that allows visualization and evaluation of
blood vessels and structural changes, where a tumor model was developed. Two induction tumor models were
investigated: tumor biopsy or cell culture. It was used a murine melanoma cell B16F10 in culture and a biopsy from a
xenograft tumor in hairless mouse. Two PS were tested: Photodithazine® and Photogem®, a chlorine and porphyrin
compounds, respectively. Using intravenous administration, the light-drug interval was of 30 minutes, 1 and 3 hours.
Illumination was performed at 630 nm and 660 nm, and the vascular and tumor response was monitored and analyzed.
The PS distribution was checked with confocal microscopy. This model can be useful to study several parameters of
PDT and the effect of this therapy in the cancer treatment since it allows direct visualization of its effects.
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Hilde Harb Buzzá, Layla Pires, Vanderlei S. Bagnato Sr., Cristina Kurachi, "Evaluation of the Photodynamic Therapy effect using a tumor model in Chorioallantoic Membrane with Melanoma cells," Proc. SPIE 8931, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII, 89310B (14 April 2014); https://doi.org/10.1117/12.2040714