Background and aims: Nanoparticles have been explored recently as an efficient delivery system
for photosensitizers in photodynamic therapy. In this study, polyhematoporphyrin (C34H38N4NaO5,)
was loaded into hollow silica nanoparticles (HSNP) by one-step wet chemical-based synthetic
route. We evaluate the efficacy and safety of polyhematoporphyrin-loaded HSNP with
hepatobiliary malignant cells and in vivo models.
Methods: Human liver cancer, cholangiocarcinoma and gallbladder cancer cells were cultured
with the HSNP and cellular viability was determined by MTT assay. Apoptotic and necrotic cells
were measured by flow cytometry. Finally, we investigate its effect in vivo.
Results: In MTT assay, the cell viability of QBC939, Huh-7, GBC-SD and HepG2 cells of the
HSNP was 6.4±1.3%, 6.5±1.2%, 3.7±1.2% and 4.7±2.0%, respectively, which were significant
different from that of free polyhematoporphyrin 62.4±4.7%, 62.5±6.0%, 33.4±6.5% and
44.3±1.9%. Flow cytometry demonstrated the laser-induced cell death with
polyhematoporphyrin-loaded HSNP was much more severe. Similarly, in vivo results of each kind
of cell revealed 14 days post-photoradiated, tumor sizes of the HSNP group were significantly
smaller. Administration of the HSNP without illumination cannot cause killing effect both in vitro
and in vivo experiments.
Conclusions: HSNP is a desirable delivery system in photodynamic therapy for hepatobiliary
malignacies, with improved aqueous solubility, stability and transport efficiency of