18 February 2014 In vitro influence of photodynamic antimicrobial chemotherapy on staphylococcus aureus by using phenothiazines derivatives associated with laser/LED light
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Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 89320H (2014) https://doi.org/10.1117/12.2038576
Event: SPIE BiOS, 2014, San Francisco, California, United States
Abstract
The aim of this study was to evaluate the effect of photodynamic antimicrobial chemotherapy (PACT) using phenothiazinium dyes - PTZ irradiated with red laser (λ660nm) or red-orange LED (λ632±2nm) on Staphylococcus aureus in vitro. triplicate tests were performed in 10 groups: control, Laser (L1+P- and L2+P-) bacterial suspensions were irradiated only with laser energy 2.4 and 4.8 J/cm2 respectively, (Led1+P- and Led2+P-) irradiated only with LED energy 2.4 and 4.8 J/cm2 respectively, (L1+P+ and L2+P+) irradiated with laser in the presence of 1μg/ml of photosensitizer, (Led1+P+ and Led2+P+) irradiated with LED in the presence of 1μg/ml of photosensitizer and finally (L-P+) only in the presence of PTZ dye. Bactericidal effect of the PACT was assessed by counting colony-forming units. The results showed no significant difference on regards different energy densities on group PACT for both lights. PACT groups (L2+P+ and Led2+P+) compared to the Control showed significant reduction of CFUs. LED/Laser groups (L2+P- and Led2+P-) compared to control and PTZ groups showed also significant differences as groups LED/Laser (4.8J/cm2) increased the average of CFUs. Although the results of this study have shown a reduction in average number of colonyforming units by the appropriate Laser or LED-dye treatment combination, it this topic requires further investigation.
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Susana C. P. S. de Oliveira, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Juliana S. C. Monteiro, Gustavo M. Pires-Santos, Gustavo M. Pires-Santos, Fernando J. P. Sampaio, Fernando J. P. Sampaio, Rafael Araújo Gomes Júnior, Rafael Araújo Gomes Júnior, Maria F. M. Gesteira, Maria F. M. Gesteira, Aldo Brugnera, Aldo Brugnera, Fátima A. A. Zanin, Fátima A. A. Zanin, Marcos A. Vannier-Santos, Marcos A. Vannier-Santos, Antônio Luiz B. Pinheiro, Antônio Luiz B. Pinheiro, } "In vitro influence of photodynamic antimicrobial chemotherapy on staphylococcus aureus by using phenothiazines derivatives associated with laser/LED light", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 89320H (18 February 2014); doi: 10.1117/12.2038576; https://doi.org/10.1117/12.2038576
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