18 February 2014 Raman and histological study of the repair of surgical bone defects grafted with biphasic synthetic micro-granular HA + β- calcium triphosphate and irradiated or not with λ780 nm laser
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Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 89320L (2014) https://doi.org/10.1117/12.2036867
Event: SPIE BiOS, 2014, San Francisco, California, United States
Abstract
The treatment of bone loss due to different etiologic factors is difficult and many techniques aim to improve repair, including a wide range of biomaterials and, recently, phototherapies. This work assessed, by Raman spectroscopy and histology, the mineralization of bone defects. Forty rats divided into 4 groups each subdivided into 2 subgroups according to the time of sacrifice were used. Bone defects were made on the femur of each animal with a trephine drill. On animals of group Clot the defect was filled only by blood clot, on group Laser the defect filled with the clot was further irradiated. On animals of groups Biomaterial and Laser + Biomaterial the defect was filled by biomaterial and the last one was further irradiated (λ780 nm, 70 mW, Φ ~ 0.4 cm2, 20 J/cm2-session, 140 J/cm2-treatment). At both 15th and 30th days following sacrifice, samples were taken and analyzed by Raman spectroscopy and light microscopy. Raman peaks of inorganic and organic contents and a more advanced stage of repair were seen on group Laser + Biomaterial. It is concluded that the use of Laser phototherapy associated to biomaterial was effective to improve the repair of bone defects.
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Antonio Luiz Barbosa Pinheiro, Antonio Luiz Barbosa Pinheiro, Luiz Guilherme P. Soares, Luiz Guilherme P. Soares, Aparecida Maria C. Marques, Aparecida Maria C. Marques, Jouber Mateus S. Aciole, Jouber Mateus S. Aciole, Jean Nunes dos Santos, Jean Nunes dos Santos, Landulfo Silveira, Landulfo Silveira, "Raman and histological study of the repair of surgical bone defects grafted with biphasic synthetic micro-granular HA + β- calcium triphosphate and irradiated or not with λ780 nm laser", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 89320L (18 February 2014); doi: 10.1117/12.2036867; https://doi.org/10.1117/12.2036867
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