Evaluation of low level laser therapy irradiation parameters on rat muscle inflammation through systemic blood cytokines

Matias Mantineo; João P. Pinheiro; António M. Morgado

doi:10.1117/12.2039342

18 February 2014 Evaluation of low level laser therapy irradiation parameters on rat muscle inflammation through systemic blood cytokines

Matias Mantineo, João P. Pinheiro, António M. Morgado

Author Affiliations +

Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 89320M (2014) https://doi.org/10.1117/12.2039342
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Low level laser therapy (LLLT) has been used for inflammation treatment. Here, we evaluate the effect of different doses, using continuous (830 and 980 nm) and pulsed illumination (830 nm), in the treatment of inflammation induced in the gastrocnemius muscle of Wistar rats, through cytokines concentration in systemic blood and histological analysis of muscle tissue. Animals were randomly divided into five groups per wavelength (5 animals per group: 10, 20, 30, 40 and 50 mW) plus a control group. LLLT was applied during five days, with constant exposure time and irradiated area (3 minutes; 0.5026 cm²). Blood was collected on days 0, 3 and 6. TNF-α, IL-1β, IL-2 and IL-6 cytokines were quantified by ELISA. Rats were killed on day 6. Muscle inflammatory cells were counted using optical microscopy. Treatment effects occurred for all applied doses (largest effect at 40 mW: 7.2 J, 14 J/cm² per irradiation), with reduction of proinflammatory TNF-α, IL-1β and IL-6 cytokines and lower number of inflammatory cells. Results were better for 830 nm. Identical methodology was used with pulsed illumination. Average power (40 mW) and duty cycle were kept constant (80%) at five frequencies (5, 25, 50, 100 and 200 Hz). Treatment effects were observed at higher frequencies, with no significant differences between them. However, the treatment effect was lower than for continuous illumination. LLLT effect on inflammation treatment can be monitored by measuring systemic blood cytokines. A larger treatment effect was observed with continuous illumination, where results seem to be compatible with a biphasic dose response.

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Matias Mantineo, João P. Pinheiro, and António M. Morgado "Evaluation of low level laser therapy irradiation parameters on rat muscle inflammation through systemic blood cytokines", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 89320M (18 February 2014); https://doi.org/10.1117/12.2039342

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