18 February 2014 Effect of LED phototherapy (λ630 ± 20nm) on mast cells during wound healing in hypothyroid
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Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 893216 (2014) https://doi.org/10.1117/12.2039880
Event: SPIE BiOS, 2014, San Francisco, California, United States
Abstract
Hypothyroidism has been associated with the disruption of the body's metabolism, including the healing process. LED phototherapy has been studied using several healing models, but their effects on mast cells proliferation associated to hypothyroidism remains unknown. The aim of this study was to assess the effect LED (λ630±20nm) phototherapy on mast cells proliferation during tissue repair in hypothyroid rats. Under general anesthesia, a standard surgical wound (1cm2) was created on the dorsum of 24 male Wistar rats divided into 4 groups of 6 animals each: EC-Control Euthyroid; ED-Euthyroid+LED; HC-Control Hypothyroid and HD-Hypothyroid+LED. The irradiation started immediately after surgery and was repeated every other day for 7 days, when animals death occurred. Hypothyroidism was induced in rats with propylthiouracil (0.05g/100mL) administered orally for 4 weeks and maintained until the end of the experiment. The specimens removed were processed to wax and stained with toluidine blue for mast cell identification. The mast cell proliferation was significantly higher in HC group than in EC group (Mann Whitney, p<0.05), but when ED group was compared to HD group, no significant difference was found. Our results showed that there was increase of mast cells in the presence of hypothyroidism, prolonging the inflammatory phase of repair, and the LED light has a biomodulative effect on mast cell population, even when hipothyroidism was present.
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Gardênia Matos Paraguassú, Isabele Cardoso Viera De Castro, Rebeca Mota Vasconcelos, Milena Goes da Guarda, Tânia Tavares Rodriguez, Maria José Pedreira Ramalho, Antônio Luiz Barbosa Pinheiro, Luciana Maria Pedreira Ramalho, "Effect of LED phototherapy (λ630 ± 20nm) on mast cells during wound healing in hypothyroid", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 893216 (18 February 2014); doi: 10.1117/12.2039880; https://doi.org/10.1117/12.2039880
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