18 February 2014 Laser photobiomodulation as an adjunct of the wound healing impairment of rats exposed to a cafeteria diet
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Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 893217 (2014) https://doi.org/10.1117/12.2040807
Event: SPIE BiOS, 2014, San Francisco, California, United States
Abstract
Obesity is associated to a delayed wound healing and prolonged inflammatory phase. Laser light has shown positive results in the photobiomodulation of tissue repair; however, its use associated with systemic disorders such as obesity is still little explored in the literature. The aim of this study was to validate an experimental system for studying weight gaining by consuming a high fat diet called "cafeteria diet" (CD) for the induction of obesity. Forty-eight rats were weaned, divided into two experimental groups: standard diet (SD) and Cafeteria Diet (CD). Free feeding was carried out during 20 weeks and the mass gaining was accompanied. After general anesthesia standardized surgical wounds were created (1cm2) in the dorsal midline region of each animal. Both groups (SD; CD) were divided into 2 subgroups of 12 animals, G1 and G3 (non-irradiated) and G2 and G4 (irradiated). The irradiation protocols (λ660 nm, 40 mW, CW; 24 J/cm2) started immediately after surgery and were repeated every other day during 14 days. The rats were killed at the 8th or 15th days after surgery. The abdominal fat was removed and weighed to verify the success of the induction technique. The specimens were taken and routinely processed histology (hematoxylin/eosin) was performed. It was concluded that the ingestion of fast-food increased abdominal fat in rats and modified the inflammatory pattern of the healing. Laser phototherapy in the parameters employed decreased inflammatory intensity quickening wound healing in obese rats.
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V. Uzeda, G. M. Paraguassu, J. N. Dos Santos, M. J. Ramalho, T. T. Rodriguez, L. M. P. Ramalho, "Laser photobiomodulation as an adjunct of the wound healing impairment of rats exposed to a cafeteria diet", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 893217 (18 February 2014); doi: 10.1117/12.2040807; https://doi.org/10.1117/12.2040807
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