4 March 2014 Flexibly combined optical microangiography and dual-wavelength laser speckle system for comprehensive imaging of hemodynamic and metabolic responses
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Abstract
We have proposed and developed a multi-modal non-invasive biomedical optical imager. It was combined from the subsystems of optical microangiography and dual-wavelength laser speckle contrast imaging. The system was designed to maintain the performances of both subsystems. It was capable of simultaneously imaging the hemodynamic and metabolic responses in tissue environment in vivo. To achieve such requirements, we utilized unique optical setup, such as paired dichroic mirrors to compensate dispersion, additional relay lens to increase working distance and translational sample probe to freely select imaging area and focal plane. The multi-functionality of the system was demonstrated in an investigation of hemodynamic and metabolic responses on an acute wound healing model in mouse pinna in vivo. The microvasculature, blood flow and hemoglobin concentration from millimeter down to capillary level were comprehensively visualized. The captured instantaneous responses to wound onset differed greatly between localized areas; after that blood flow had a rebalance tendency, and hemoglobin concentration dynamically recovered to baseline situation.
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Lei Shi, Lei Shi, Jia Qin, Jia Qin, Lin An, Lin An, Ruikang K. Wang, Ruikang K. Wang, } "Flexibly combined optical microangiography and dual-wavelength laser speckle system for comprehensive imaging of hemodynamic and metabolic responses", Proc. SPIE 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII, 89341F (4 March 2014); doi: 10.1117/12.2041152; https://doi.org/10.1117/12.2041152
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