4 March 2014 Rotational imaging OCT for full-body embryonic imaging
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Abstract
Optical coherence tomography (OCT) has proved to be an effective tool to study the development of mammalian embryos due to its high resolution and contrast. However, light attenuation is an important factor which constrains the imaging depth of OCT. Limitation of imaging depth will inhibit us to better study the structural characteristics of mouse embryos. Here we propose a new method, rotational imaging OCT (riOCT), to improve the imaging depth and provide full-body embryonic imaging. The experimental setup comprises the swept source OCT system and the square glass tube mounted on a rotational stage. The E10.5 mouse embryos are dissected and immersed in the glass tube using 0.9% saline solution. 3D structural imaging is performed at four different angles with the interval of 90 degrees. The OCT image records the optical distances of different components such as glass, gelatin and tissue. The position of rotation center is determined by the track of the glass tube center at different angles. The final image is acquired by rotating the images at different angles according to the rotation center. Results indicate that this method is able to improve the visualization of structural information of mouse embryo compared to conventional OCT.
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Narendran Sudheendran, Chen Wu, Irina V. Larina, Mary E. Dickinson, Kirill V. Larin, "Rotational imaging OCT for full-body embryonic imaging", Proc. SPIE 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII, 89342K (4 March 2014); doi: 10.1117/12.2041990; https://doi.org/10.1117/12.2041990
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