Fluorescence spectroscopy using indocyanine green for lymph node mapping

Neda Haj-Hosseini; Pascal Behm; Ivan Shabo; Karin Wårdell

doi:10.1117/12.2036765

27 February 2014 Fluorescence spectroscopy using indocyanine green for lymph node mapping

Neda Haj-Hosseini, Pascal Behm, Ivan Shabo, Karin Wårdell

Proceedings Volume 8935, Advanced Biomedical and Clinical Diagnostic Systems XII; 893504 (2014) https://doi.org/10.1117/12.2036765
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The principles of cancer treatment has for years been radical resection of the primary tumor. In the oncologic surgeries where the affected cancer site is close to the lymphatic system, it is as important to detect the draining lymph nodes for metastasis (lymph node mapping). As a replacement for conventional radioactive labeling, indocyanine green (ICG) has shown successful results in lymph node mapping; however, most of the ICG fluorescence detection techniques developed are based on camera imaging. In this work, fluorescence spectroscopy using a fiber-optical probe was evaluated on a tissue-like ICG phantom with ICG concentrations of 6-64 μM and on breast tissue from five patients. Fiber-optical based spectroscopy was able to detect ICG fluorescence at low intensities; therefore, it is expected to increase the detection threshold of the conventional imaging systems when used intraoperatively. The probe allows spectral characterization of the fluorescence and navigation in the tissue as opposed to camera imaging which is limited to the view on the surface of the tissue.

Citation Download Citation

Neda Haj-Hosseini, Pascal Behm, Ivan Shabo, and Karin Wårdell "Fluorescence spectroscopy using indocyanine green for lymph node mapping", Proc. SPIE 8935, Advanced Biomedical and Clinical Diagnostic Systems XII, 893504 (27 February 2014); https://doi.org/10.1117/12.2036765

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