4 March 2014 Electrochemical impedance spectroscopy based-on interferon-gamma detection
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Abstract
Tuberculosis (TB) is an ancient disease constituted a long-term menace to public health. According to World Health Organization (WHO), mycobacterium tuberculosis (MTB) infected nearly a third of people of the world. There is about one new TB occurrence every second. Interferon-gamma (IFN-γ) is associated with susceptibility to TB, and interferongamma release assays (IGRA) is considered to be the best alternative of tuberculin skin test (TST) for diagnosis of latent tuberculosis infection (LTBI). Although significant progress has been made with regard to the design of enzyme immunoassays for IFN-γ, adopting this assay is still labor-intensive and time-consuming. To alleviate these drawbacks, we used IFN-γ antibody to facilitate the detection of IFN-γ. An experimental verification on the performance of IGRA was done in this research. We developed two biosensor configurations, both of which possess high sensitivity, specificity, and rapid IFN-γ diagnoses. The first is the electrochemical method. The second is a circular polarization interferometry configuration, which incorporates two light beams with p-polarization and s-polarization states individually along a common path, a four photo-detector quadrature configuration to arrive at a phase modulated ellipsometer. With these two methods, interaction between IFN-γ antibody and IFN-γ were explored and presented in detail.
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Guan-Wei Li, Guan-Wei Li, Yi-Ching Kuo, Yi-Ching Kuo, Pei-I Tsai, Pei-I Tsai, Chih-Kung Lee, Chih-Kung Lee, } "Electrochemical impedance spectroscopy based-on interferon-gamma detection", Proc. SPIE 8936, Design and Quality for Biomedical Technologies VII, 893606 (4 March 2014); doi: 10.1117/12.2039188; https://doi.org/10.1117/12.2039188
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