17 February 2014 Validation of temperature-modulated fluorescence tomography in vivo
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Proceedings Volume 8937, Multimodal Biomedical Imaging IX; 89370H (2014) https://doi.org/10.1117/12.2040878
Event: SPIE BiOS, 2014, San Francisco, California, United States
Abstract
To overcome the strong scattering in biological tissue that has long afflicted fluorescence tomography, we have developed a novel technique, "temperature-modulated fluorescence tomography" (TM-FT) to combine the sensitivity of fluorescence imaging with focused ultrasound resolution. TM-FT relies on two key elements: temperature sensitive ICG loaded pluronic nanocapsules we termed ThermoDots and high intensity focused ultrasound (HIFU). TM-FT localizes the position of the fluorescent ThermoDots by irradiating and scanning a HIFU beam across the tissue while conventional fluorescence tomography measurements are acquired. The HIFU beam produces a local hot spot, in which the temperature suddenly increases changing the quantum efficiency of the ThermoDots. The small size of the focal spot (~1 mm) up to a depth of 6 cm, allows imaging the distribution of these temperature sensitive agents with not only high spatial resolution but also high quantitative accuracy in deep tissue using a proper image reconstruction algorithm. Previously we have demonstrated this technique with a phantom study with ThermoDots sensitive in the 20-25°C range. We recently optimized the ThermoDots for physiological temperatures. In this work, we will demonstrate a new HIFU scanning method which is optimized for in vivo studies. The performance of the system is tested using a phantom that resembles a small animal bearing a small tumor targeted by ThermoDots.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tiffany C. Kwong, Tiffany C. Kwong, Farouk Nouizi, Farouk Nouizi, Yuting Lin, Yuting Lin, Rushi Rajyaguru, Rushi Rajyaguru, Trinh Nguyen, Trinh Nguyen, Lara Alptekin, Lara Alptekin, Uma Sampathkumaran, Uma Sampathkumaran, Yue Zhu, Yue Zhu, Shaaz Ahmed, Shaaz Ahmed, Gultekin Gulsen, Gultekin Gulsen, } "Validation of temperature-modulated fluorescence tomography in vivo", Proc. SPIE 8937, Multimodal Biomedical Imaging IX, 89370H (17 February 2014); doi: 10.1117/12.2040878; https://doi.org/10.1117/12.2040878
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