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17 February 2014 Validation of temperature-modulated fluorescence tomography in vivo
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Proceedings Volume 8937, Multimodal Biomedical Imaging IX; 89370H (2014)
Event: SPIE BiOS, 2014, San Francisco, California, United States
To overcome the strong scattering in biological tissue that has long afflicted fluorescence tomography, we have developed a novel technique, "temperature-modulated fluorescence tomography" (TM-FT) to combine the sensitivity of fluorescence imaging with focused ultrasound resolution. TM-FT relies on two key elements: temperature sensitive ICG loaded pluronic nanocapsules we termed ThermoDots and high intensity focused ultrasound (HIFU). TM-FT localizes the position of the fluorescent ThermoDots by irradiating and scanning a HIFU beam across the tissue while conventional fluorescence tomography measurements are acquired. The HIFU beam produces a local hot spot, in which the temperature suddenly increases changing the quantum efficiency of the ThermoDots. The small size of the focal spot (~1 mm) up to a depth of 6 cm, allows imaging the distribution of these temperature sensitive agents with not only high spatial resolution but also high quantitative accuracy in deep tissue using a proper image reconstruction algorithm. Previously we have demonstrated this technique with a phantom study with ThermoDots sensitive in the 20-25°C range. We recently optimized the ThermoDots for physiological temperatures. In this work, we will demonstrate a new HIFU scanning method which is optimized for in vivo studies. The performance of the system is tested using a phantom that resembles a small animal bearing a small tumor targeted by ThermoDots.
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Tiffany C. Kwong, Farouk Nouizi, Yuting Lin, Rushi Rajyaguru, Trinh Nguyen, Lara Alptekin, Uma Sampathkumaran, Yue Zhu, Shaaz Ahmed, and Gultekin Gulsen "Validation of temperature-modulated fluorescence tomography in vivo", Proc. SPIE 8937, Multimodal Biomedical Imaging IX, 89370H (17 February 2014); doi: 10.1117/12.2040878;

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