20 February 2014 Optical fiber spectroscopy measures perfusion of the brain in a murine Alzheimer's disease model
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Abstract
Optical fiber spectroscopy is a versatile tool for measuring diffuse reflectance and extracting absorption information that can noninvasively quantify the presence of chromophores such as oxyhemoglobin and deoxy-hemoglobin in tissues. Cerebrovascular abnormalities were widely recognized in Alzheimer’s disease (AD) patients. We analyzed blood volume fraction and level of oxygenated hemoglobin in Tg6799 mice, which are transgenic mice expressing five different familial Alzheimer disease-associated mutations in the human amyloid precursor protein and presenilin-1 genes. Diffuse reflectance spectra were iteratively fit as weighted sums of oxy- and deoxy-hemoglobin. Our observations showed slightly hypoxic conditions and significantly increased blood volume in the Alzheimer’s mice versus wild type. These results suggest that hyperperfusion of our AD mice may be a compensating mechanism for impaired cerebral vascular function and somehow relevant with early stage of AD patients. Ongoing work focuses on developing a cannula fixture that allows measurement in awake, behaving animals.
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Hyung Jin Ahn, Hyung Jin Ahn, Sidney Strickland, Sidney Strickland, James Krueger, James Krueger, Daniel Gareau, Daniel Gareau, } "Optical fiber spectroscopy measures perfusion of the brain in a murine Alzheimer's disease model", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 89380Q (20 February 2014); doi: 10.1117/12.2040287; https://doi.org/10.1117/12.2040287
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