20 February 2014 Bio-functionalized hollow core photonic crystal fibers for label-free DNA detection
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Abstract
Bio-functionalization of inner surfaces of all silica Hollow Core–Photonic Crystal Fibers (HC-PCF) has been investigated. The approach is based on layer-by-layer self-assembly Peptide Nucleic Acid (PNA) probes, which is an oligonucleotide mimic that is well suited for specific DNA target recognition. Two kinds of HC-PCFs have been considered: a photonic Bragg fiber and a hollow core (HC-1060) fiber. After spectral characterization and internal surface functionalization by using PNA probes, genomic DNA solutions from soy flour were infiltrated into the fibers. The experimental results indicate that hybridization of the complementary strand of target DNA increases the thickness of the silica layer and leads up to the generation of surface modes, resulting in a significant modulation of the transmission spectra. Numerical analysis confirms such behavior, suggesting the possibility to realize biological sensing.
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A. Candiani, Hussein T. Salloom, E. Coscelli, M. Sozzi, A. Manicardi, Ahmad K. Ahmad, A. Hadi Al-Janabi, R. Corradini, G. Picchi, A. Cucinotta, S. Selleri, "Bio-functionalized hollow core photonic crystal fibers for label-free DNA detection", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 89380T (20 February 2014); doi: 10.1117/12.2039665; https://doi.org/10.1117/12.2039665
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