20 February 2014 Biconically tapered fiber optic dip probe for rapid label-free immunoassays
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We report U-shaped biconically tapered optical fibers (BTOF) as dip probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 0.5, 5.0, and 50 μg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change in the sensor signal, which allows real-time monitoring. The kinetics of the antibody (mouse Ig-G) -antigen (rabbit anti-mouse IgG) reactions was studied. The limit of detection for the sensor was estimated to be less than 0.5 μg/mL with low temperature sensitivity. Utilization of the rate of the sensor peak shift within the first few minutes of antibody-antigen reaction is proposed as a rapid detection method.
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John Miller, John Miller, Angelica Castaneda, Angelica Castaneda, Kun Ho Lee, Kun Ho Lee, Martin Sanchez, Martin Sanchez, Shelton Murinda, Shelton Murinda, Wei-Jen Lin, Wei-Jen Lin, Ertan Salik, Ertan Salik, "Biconically tapered fiber optic dip probe for rapid label-free immunoassays", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893815 (20 February 2014); doi: 10.1117/12.2037987; https://doi.org/10.1117/12.2037987

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