20 February 2014 Application of ball-lens hollow fiber Raman probe for studying anorectal prolapse
Author Affiliations +
Abstract
Our Raman probe that is called as ball-lens hollow fiber Raman probe (BHRP) had been proved possessing capability to detect the biochemical alteration within biological tissue. Whether BHRP has high capability and sensitivity in diagnosing the biochemical changing of tissue or not, mouse's normal rectal and anorectal prolapse (AP) were decided to be used as a model for this non invasive method. This AP is azoxymethane and DSS-induced mouse’s anorectal prolapse. Main outcome of BHRP will be potential for non-invasive method in tumor diagnosing. BHRP spectra obtained were a high quality and allowed analysis of their differences between normal rectal (control group) and AP. After spectral acquisition and comparison with corresponding images of hematoxylin/eosinstained section observation used to make the histopathologic diagnosing, BHRP detected some differences within the region of moiety of DNA, protein (i.e. collagen) and lipid, then following with the alteration of symmetric P=O stretching vibration compared with the normal rectal tissue. BHRP discriminate normal tissue and AP in the real-time.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Bibin Bintang Andriana, Bibin Bintang Andriana, Akinori Taketani, Akinori Taketani, C. Linda R. Soeratman, C. Linda R. Soeratman, Mika Ishigaki, Mika Ishigaki, Yasuhiro Maeda, Yasuhiro Maeda, Masanori Sawa, Masanori Sawa, Hidetoshi Sato, Hidetoshi Sato, } "Application of ball-lens hollow fiber Raman probe for studying anorectal prolapse", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893816 (20 February 2014); doi: 10.1117/12.2037884; https://doi.org/10.1117/12.2037884
PROCEEDINGS
11 PAGES


SHARE
Back to Top